Research Paper Volume 12, Issue 19 pp 19045—19059

FGF23 protects osteoblasts from dexamethasone-induced oxidative injury


FGF23 inhibits DEX-induced oxidative stress in osteoblasts. OB-6 osteoblastic cells (AC) or the primary murine osteoblasts (DF) were pretreated with applied concentration of FGF23 (5 or 25 ng/mL) or vehicle control (PBS) for 2h, followed by dexamethasone (DEX, 1 μM) stimulation, cells were further cultured for additional 12h, superoxide contents (A and D), lipid peroxidation intensity (B and E) and mitochondrial depolarization (JC-1 green fluorescence accumulation, C and F) were tested, with results normalized. Data were mean ± standard deviation (SD, n=5). “Veh” stands for vehicle control for DEX. * p<0.05 vs. “Veh” cells with PBS pretreatment. # p<0.05 vs. DEX-treated cells with PBS pretreatment. Each experiment was repeated three times and similar results were obtained.