Research Paper Volume 13, Issue 4 pp 4976—4985

GPR173 agonist phoenixin 20 promotes osteoblastic differentiation of MC3T3-E1 cells

The effects of phoenixin 20 in osteoblast differentiation are dependent on GPR173. Cells were transfected with siGPR173 or non-specific siRNA; then cultured in osteogenic medium containing phoenixin 20 (15 nM) with for 14 days. (A) Successful knockdown of GPR173; (B) Levels of RUNX-2; (C) Alizarin Red S staining; (D) ALP activity (*, #, P

Figure 7. The effects of phoenixin 20 in osteoblast differentiation are dependent on GPR173. Cells were transfected with siGPR173 or non-specific siRNA; then cultured in osteogenic medium containing phoenixin 20 (15 nM) with for 14 days. (A) Successful knockdown of GPR173; (B) Levels of RUNX-2; (C) Alizarin Red S staining; (D) ALP activity (*, #, P<0.01, n=4-5).