Research Paper Volume 12, Issue 20 pp 20163—20183

Down-regulated ciRS-7/up-regulated miR-7 axis aggravated cartilage degradation and autophagy defection by PI3K/AKT/mTOR activation mediated by IL-17A in osteoarthritis

Figure 2. IL-1β exposure induced defection of autophagy in chondrocytes. (A) Western blot analysis of IL-1β-induced chondrocytes at different time points (6, 12, 24, 36, and 48 h) using antibodies directed to LC3, p62, and Beclin1 and normalized to β-actin. Data represent the mean ± SD (n=5), ** p < 0.01 and *** p < 0.001 vs. unstimulated cells; (B) Representative immunofluorescence photomicrograph of LC3 (red)-labelled chondrocytes. Nuclei were stained with DAPI (blue).