Research Paper Volume 12, Issue 15 pp 15532—15545

circIQCH sponges miR-145 to promote breast cancer progression by upregulating DNMT3A expression

Figure 2. Downregulation of circIQCH suppresses the proliferation of breast cancer cells. (A) Knock down of circIQCH was assessed by qRT-PCR analysis. (B) si-circIQCH decreased the expression of circIQCH while had no effect on linear IQCH mRNA. (C, D) CCK-8 assays detected cell proliferation. (E) Mouse xenograft models were established. (F) Tumor volume were estimated in every four days. (G) The xenograft tumors were analyzed by immunohistochemistry analysis, and the representative images of ki-67 expression are presented. *P<0.05; **P<0.01.