Figure 3. Effects of IL-6 KO on cardiac remodeling and aging-related protein levels. (A–C) Body weights (BWs), heart weights (HWs), and HW/BW ratios in the four groups. (D) MC CSA and cardiac fibrosis area in each group. (E) Lipofuscin density in each group. (F) p16, p19, p21, p53, and Sirt1 mRNA expression levels were analyzed by RT-PCR. (G) Cardiac p53 protein expression was detected by immunofluorescence staining. N=5-8 in each group. * p<0.05 vs. the young WT group; # p<0.05 vs. the aging WT group.