Research Paper Volume 12, Issue 15 pp 15546—15555

Glycochenodeoxycholic acid induces stemness and chemoresistance via the STAT3 signaling pathway in hepatocellular carcinoma cells

Figure 1. GCDC enhances chemoresistance in HCC cells. (A–D) Cell viability was detected using the CCK-8 assay. *P<0.05, **P<0.01. (E–H) Cell apoptosis was analyzed by flow cytometry. *P<0.05. (I–J) GCDC enhanced the ability of chemoresistance of HCC cells in vivo. Huh7 cells (5×106) were pretreated with GCDC and then were implanted in the right subcutaneous armpit area of nude mice. Then the cisplatin (4mg/kg) were injected in tumor every 3 days. After 27 days, the mice were sacrificed and the weight of the tumor was measured.*P<0.05, **P<0.01.