Research Paper Volume 12, Issue 15 pp 15546—15555

Glycochenodeoxycholic acid induces stemness and chemoresistance via the STAT3 signaling pathway in hepatocellular carcinoma cells

Figure 5. GCDC promotes chemoresistance of HCC through the STAT3 signaling pathway. (A) Western blotting was used to assess the effect of siRNA on the STAT3 expression. P84 was used as an internal reference. (B, C) Cell viability was detected by the CCK-8 assay. *P<0.05, **P<0.01. (D, E) Cell apoptosis was analyzed using flow cytometry. *P<0.05. (F) The expression of apoptotic and anti-apoptotic genes in Huh7 cells was examined by reverse-transcriptase-polymerase chain reaction (RT-PCR) assays. **P<0.01, ***P<0.001.