NLRP3 was highly expressed in LPS-induced NR8383 cell and ALI mouse models. NLRP3 expression in NR8383 cells and mice tissues with or without LPS induction assessed by RT-qPCR (N = 10) (A). Levels of NLRP3, pro-caspase-1/caspase-1 and pro-IL-1β/IL-1β normalized to GAPDH in alveolar macrophages (NR8383) of normal mice and mice with ALI evaluated by Western blot analysis (N = 10) (B). Interference efficiency of NLRP3 normalized to GAPDH evaluated by Western blot analysis (N = 10) (C). Levels of cytokines IL-18 and IL-1β detected by ELISA (D). Levels of caspase-1, TNF-α and IL-6 protein normalized to GAPDH in NR8383 cells by Western blot analysis (E). * p < 0.05 vs. PBS + NR8383, normal or si-NC. Measurement data were expressed as mean ± standard deviation. Comparison between two groups was conducted using unpaired t-test. The experiments were repeated three times independently.