Research Paper Volume 12, Issue 20 pp 20198—20211

Silencing of long non-coding RNA MEG3 alleviates lipopolysaccharide-induced acute lung injury by acting as a molecular sponge of microRNA-7b to modulate NLRP3

Figure 4. LncRNA MEG3 sponged miR-7b through NLRP3 to modulate the LPS-induced ALI cells in vitro. The expression of NLRP3 normalized to GAPDH assessed by Western blot analysis (A). The levels of cytokines IL-18 and IL-1β detected by ELISA (B). The expression of caspase-1, TNF-α and IL-6 normalized to GAPDH detected by Western blot analysis (C). * p < 0.05 vs. si-MEG3 + NC mimic; # p < 0.05 vs. si-MEG3 + oe-NC. Measurement data were expressed as mean ± standard deviation. Data among multiple groups were tested using ANOVA, followed by Tukey’s post hoc test. The experiments were repeated three times independently.