Research Paper Volume 12, Issue 21 pp 21129—21146

LINC00452 promotes ovarian carcinogenesis through increasing ROCK1 by sponging miR-501-3p and suppressing ubiquitin-mediated degradation

Figure 2. LINC00452 promotes ovarian cancer cell carcinogenic properties. (A) qPCR analysis displaying mRNA expression of LINC00452, miR-501-3p and ROCK1 in a panel of ovarian cancer cell lines and normal ovary epithelial cell line IOSE80. (B) RNA FISH results showing that LINC00452 was mainly located in the cytoplasm of CaOV3 cells. U6 was used as a control for nuclear localization. (C) Validation of LINC00452 expression in LINC00452-overexpressing and knockdown CaOV3 cells by qPCR. *** p < 0.001 versus control cells, one-way ANOVA test. (D) Time-dependent cell proliferation determined by MTT assay. LINC00452-overexpressing cells exhibited a growth advantage while LINC00452-knockdown cells displayed a growth defect over control CaOV3 cells. ** p < 0.01, *** p < 0.001 versus control cells, one-way ANOVA test. (E) Cell migration/invasion capacity determined by transwell assay. Upregulation of LINC00452 promoted whereas downregulation of LINC00452 compromised cell migration and invasion of CaOV3 cells. ** p < 0.01 versus control cells, one-way ANOVA test. (F) Colony-formation assay showing enhanced cell growth upon increasing LINC00452 and defective cell growth upon decreasing its expression. ** p < 0.01 versus control cells, one-way ANOVA test.