Figure 4. Luciferase assay was used to validate targeting relationships. (A-C) Expression levels of LINC00265, miR-382-5p, SAT1 and VAV3 in osteosarcoma cells and osteoblasts. (D) Transfection efficiency of OE-LINC00265 and sh-LINC00265. (E, F) Luciferase assay confirmed that LINC00265 targeted miR-382-5p. (G) Transfection efficiency of miR-382-5p inhibitor and miR-382-5p mimic. (H-J) Luciferase assay confirmed that miR-382-5p targeted SAT1 and VAV3. *P < 0.05 vs. sh-NC or inhibitor-NC group; #P < 0.05 vs. OE-NC or mimic-NC group.