Research Paper Volume 12, Issue 19 pp 19293—19315

Modification of Mcl-1 alternative splicing induces apoptosis and suppresses tumor proliferation in gastric cancer


Altered myeloid cell leukemia (Mcl)-1 splicing from Mcl-1L to Mcl-1S promotes apoptosis in vivo. (A) Hematoxylin and eosin staining of tumor sections of the xenograft models is shown. The red arrows indicate dead cells. The dead cell area/overall tumor area increased in a dose-dependent manner after steric-blocking oligonucleotide (SBO) treatment. (B) Immunofluorescence with annexin V staining (the white arrows indicate green fluorescence) showing apoptotic cells in the tumor sections of the HGC-27 xenograft models is presented. The results of the between-group and repeated-measure analyses are shown as the means ± standard deviation (SD). (C) Flow cytometry showing the apoptosis rates of tumor cells treated with SBOs at indicated dosages is presented. Between-group comparison results are shown as the means ± SD.