Research Paper Volume 12, Issue 20 pp 20235—20253

ARHGEF11 promotes proliferation and epithelial-mesenchymal transition of hepatocellular carcinoma through activation of β-catenin pathway

Figure 2. Knockdown of ARHGEF11 induces cell growth inhibition, cell cycle arrest and cell apoptosis in hepatoma cell lines. (A) ARHGEF11 knockdown with two different siRNAs (siARHGEF11-1, siARHGEF11-1) or negative control (siNC) as examined by western blot and qRT-PCR. (B) The viability of hepatoma cells detected by CCK8 assay. (C) Cell cycle distribution detected by flow cytometry. The representative images (left) and percentage of cell cycle (right) are shown. (D) Cell apoptosis detected by flow cytometry. The representative images (left) and relative percentage of apoptotic cells (right) are shown. Data are shown as mean ± SD. *P<0.05, **P<0.01.