Figure 2. Knockdown of ARHGEF11 induces cell growth inhibition, cell cycle arrest and cell apoptosis in hepatoma cell lines. (A) ARHGEF11 knockdown with two different siRNAs (siARHGEF11-1, siARHGEF11-1) or negative control (siNC) as examined by western blot and qRT-PCR. (B) The viability of hepatoma cells detected by CCK8 assay. (C) Cell cycle distribution detected by flow cytometry. The representative images (left) and percentage of cell cycle (right) are shown. (D) Cell apoptosis detected by flow cytometry. The representative images (left) and relative percentage of apoptotic cells (right) are shown. Data are shown as mean ± SD. *P<0.05, **P<0.01.