Research Paper Volume 12, Issue 20 pp 20235—20253

ARHGEF11 promotes proliferation and epithelial-mesenchymal transition of hepatocellular carcinoma through activation of β-catenin pathway

Figure 6. Knockdown of ARHGEF11 attenuates EMT, cell cycle progression and induces cell apoptosis via β-catenin-dependent way. (A) Micrographs of indicated cell clones. (B) The mRNA expression of a series of EMT markers and inducers in indicated cells. (C) Relative expression of E-cadherin, N-cadherin, Vimentin and ZEB1 detected by western blot in the indicated cells. (D) Immunofluorescence assay of E-cadherin, Vimentin, and ZEB1 expression in the indicated cells. The merged images show overlays of ZEB1 (red) and nuclear staining by DAPI (blue); scalebar: 20 mm. (E) The mRNA levels of CDK1, CDK2, cyclinD1 and cyclinE in indicated cells (F) Relative expression of cyclinD1 and Cleaved Caspase-3 detected by western blot in the indicated cells.