Figure 5. Effects of Tim-3 knockdown on Tim-3 protein levels and neurocyte apoptosis after SAH. Western blotting showing that Tim-3 siRNA significantly decreased the expression of Tim-3 (A) as assessed by quantitative analysis (B). Immunofluorescence staining also verified that Tim-3 siRNA significantly decreased Tim-3expression (C). Representative TUNEL staining images (D) and quantitative analysis of TUNEL-positive cells (E) in the ipsilateral cortex after SAH with Tim-3 siRNA or scramble siRNA treatment (n = 6 in each group). Data are expressed as mean ± SD. **p < 0.01, ***p < 0.001.