Research Paper Volume 12, Issue 21 pp 21161—21185

Tim-3 deteriorates neuroinflammatory and neurocyte apoptosis after subarachnoid hemorrhage through the Nrf2/HMGB1 signaling pathway in rats

Figure 8. NK252 treatment reversed the effect of Tim-3 on the expression of HMGB1 and Nrf2. Compared with the SAH+AAV-Tim-3+DMSO groups, western blotting data indicated that NK252 significantly decreased the expression of HMGB1 and increased the expression of Nrf2 (A) as assessed by quantitative analysis of HMGB1 (B) and Nrf2 (C) in the left hemisphere at 24 h post-SAH(n = 6 in each group). Data are expressed as the mean ± SEM.**p < 0.01,***p < 0.001. Representative microphotographs of immunofluorescence staining for HMGB1 and Nrf2 (D). Scale bar = 50 μm. Immunohistochemical staining of HMGB1 and Nrf2 (E) in the left cerebral cortex at 24 h post-SAH. Scale bar = 100 μm.