Research Paper Volume 12, Issue 20 pp 20285—20307

LncRNA DGCR5 plays a tumor-suppressive role in glioma via the miR-21/Smad7 and miR-23a/PTEN axes

Figure 3. NF-κB1 binds to the promoter region of lncRNA DGCR5 to inhibit its expression. (A, B) NF-κB1 overexpression or silencing generated in U251-MG and SHG44 cells by transfection of NF-κB1-overexpressing vector or si-NF-κB1, as confirmed by immunoblotting and real-time PCR. (C) DGCR5 expression in response to NF-κB1 overexpression or silencing determined by real-time PCR. (D) Wild-type and mutant-type psicheck-2-proDGCR5 luciferase reporter vectors were constructed as described in the Materials and methods section. These vectors were cotransfected in 293T cells with pcDNA3.1/NF-κB1 and the luciferase activity was determined. *P<0.05, **P<0.01, compared to the si-NC group. ##P<0.01, compared to vector group.