Research Paper Volume 12, Issue 20 pp 20285—20307

LncRNA DGCR5 plays a tumor-suppressive role in glioma via the miR-21/Smad7 and miR-23a/PTEN axes

Figure 4. Effects of lncRNA DGCR5 overexpression on glioma cells. (A) LncRNA DGCR5 overexpression or silencing was generated in U251-MG and SHG44 cells by transfection of lncRNA DGCR5-overexpressing vector (lncDGCR5) or small hairpin RNA (sh-DGCR5), as confirmed by real-time PCR. Next, DNA synthesis capacity was determined by EdU assay (B); cell apoptosis was determined by Flow cytometry assay (C); cell invasive capacity was determined by Transwell assay (D); cell migratory capacity was determined by Wound healing assay (E). (F) U251-MG and SHG44 cells were transfected with lncDGCR5 or sh-DGCR5 and the protein levels of E-cadherin, VIM, Snai2, and TWIST were determined by immunoblotting. *P<0.05, **P<0.01, ***P<0.005 compared to vector group; ## P<0.01 compared to sh-NC group.