Figure 6. DGCR5/miR-21 axis affect glioma cell migration and invasion through Smad7. (A) U251-MG and SHG44 cells were transfected with lncRNA DGCR5-overexpressing vector and examined for the protein levels of Smad7 by immunoblotting. Next, U251-MG and SHG44 cells were co-transfected with DGCR5 and miR-21 mimic and examined for (B) migratory capacity by wound healing assay; (C) invasive capacity by Transwell assay; (D) the protein levels of Smad7, E-cadherin, VIM, Snai2, and TWIST were determined by immunoblotting. *P<0.05, **P<0.01, compared to the control group; #P<0.05, ##P<0.01, compared to the vector + miR-21 mimic group.