Research Paper Volume 12, Issue 20 pp 20308—20331

TRIM28 is a distinct prognostic biomarker that worsens the tumor immune microenvironment in lung adenocarcinoma

Figure 3. Analysis of DEGs according to the immune and stromal scores in LUAD patients. (A, B) Clustering Heatmap plot of the DEGs. The upper color bar represents the sample class; red represents the group with higher scores, while blue represents the group with lower scores. Genes with higher levels are shown in red, while those with lower levels are shown in green. (C, D) Venn diagrams showing the number of commonly upregulated (C) or downregulated (D) DEGs. (E) PPI analysis of downregulated DEGs via STRING. The interaction score was set to medium confidence (0.400). (F, G) The top 15 GO enrichment terms (F) and KEGG enrichment terms (G) for all DEGs, analyzed in Metascape. (H) The first 15 genes identified through the maximal clique centrality method were chosen as hub genes using the cytoHubba plugin. More red color represents more forward ranking. GO, gene ontology; KEGG, Kyoto Encyclopedia of Genes and Genomes; DEGs, differentially expressed genes; LUAD, lung adenocarcinoma; MMC, maximal clique centrality.