Figure 8. The association of TRIM28 with IRF5 and IRF8. (A) Network view of predicted E3-substrate interactions in UbiBrowser web services. In network view, the central node is the queried E3 ligase, and the surrounding nodes are the predicted substrates. The width of the edge reflects the confidence of the interaction. (B) The possible binding regions of IRF5 and IRF8 to TRIM28. (C–H) TRIM28 expression exhibited a significant negative correlation with IRF5 and IRF8 expression in TCGA (C, D), GSE43580 (E, F) and GSE7670 (G, H). (I–N) TRIM28 expression exhibited a strong negative correlation with stromal scores (I) and immune scores (J), while IRF5 and IRF8 levels exhibited strong positive relationships with stromal scores (K, M) and immune scores (L, N), respectively. (O) The expression of IRF5 and IRF8 after knocking down TRIM28 in two different LUAD cell lines (PC9 and H1299) through Western blotting. The gels have been run under the same experimental conditions. The blot of IRF5 in H1299 is obtained from the combined image merging the blot image and the ladder image. LUAD, lung adenocarcinoma; TCGA, The Cancer Genome Atlas; GSE, Gene Expression Omnibus data series.