Research Paper Volume 12, Issue 15 pp 15756—15770

MiR-483 induces senescence of human adipose-derived mesenchymal stem cells through IGF1 inhibition

Figure 3. Effects of miR-483 on adipogenic differentiation of hADSCs. (A, B) The efficiency of miR-483-5p and miR-483-3p inhibition was assayed by RT-qPCR. (C) The effect of inhibition of miR-483-3p and miR-483-5p on adipogenic differentiation of hADSCs was evaluated on day 14 using Oil Red O staining (10×; scale: 50μm). (DF) RT-qPCR and western blot analysis of LPL and PPARγ expression levels. I-CT: negative control inhibitor; 483-5p-I: inhibitor of miR-483-5p; 483-3p-I: inhibitor of miR-483-3p.