Research Paper Volume 12, Issue 15 pp 15756—15770

MiR-483 induces senescence of human adipose-derived mesenchymal stem cells through IGF1 inhibition

Figure 4. Identification of IGF1 as a direct target of miR-483-3p. (A) Putative miR-483-3p target sites in IGF1 and design of mutation sites. (B) Activity of the luciferase reporter gene in HEK-293T cells co-transfected with a luciferase reporter plasmid containing IGF1 3′ UTRs and wild-type pSUPER-miR-483-3p, mutant pSUPER-miR-483-3p, or empty pSUPER vector. Luciferase activity was assayed 32 hours after transfection. (C, D) RT-qPCR analysis of IGF1 mRNA levels in hADSCs transfected with 483-3p-M or 483-3p-I. mut: mutant; M-CT: mimic control; 483-3p-M: mimic of miR-483-3p.