Research Paper Volume 12, Issue 23 pp 23668—23683

DNMT3B silencing suppresses migration and invasion by epigenetically promoting miR-34a in bladder cancer


Figure 4. DNMT3B knockdown inhibits migration, invasion, and EMT in bladder cancer cells. EJ and UMUC3 cells were infected with lentiviral vectors expressing shRNAs targeting DNMT3B (shRNA)to construct DNMT3B stable knockdown cells, termed as shRNA group. EJ and UMUC3 cells infected with empty lentivirus were used as the NC group. (A, B) The wound healing assay (A) and the Transwell migration assay (B) were performed to evaluate the migration capability. (C) The Transwell invasion assay was performed to evaluate the invasion capability. Data were presented as mean±SD. *p<0.05 between NC and shRNA. (D) Expression of epithelial-mesenchymal transition markers as detected by western blot analysis. DNMT3B, DNA methyltransferase 3B; EMT, epithelial-mesenchymal transition; NC, negative control; SD, standard deviation.