Research Paper Advance Articles pp 23668—23683

DNMT3B silencing suppresses migration and invasion by epigenetically promoting miR-34a in bladder cancer

Figure 5. The miR-34a inhibitor reduced the effects of DNMT3B knockdown on migration, invasion, and EMT. The shRNA groups of EJ and UMUC3 cells were transfected with the miR-34a inhibitor (shRNA+inhibitor) or the negative control miRNA (shRNA+miR-NC). The NC groups were used as the control. (A) The miR-34a level was confirmed by qRT-PCR. (B, C) The wound healing assay (B) and the Transwell migration assay (C) were performed to evaluate the migration capability of each group of cells. (D) The Transwell invasion assay was performed to evaluate the invasion capability of each group of cells. (E) The expression of the EMT markers was assessed using western blot analysis, using GAPDH as the loading control. Data were presented as means±SD. *p<0.05 between shRNA+miR-NC and shRNA+inhibitor. DNMT3B, DNA methyltransferase 3B; EMT, epithelial-mesenchymal transition; NC, negative control; qRT-PCR, quantitative reverse transcription polymerase chain reaction; SD, standard deviation.