Research Paper Volume 12, Issue 19 pp 19375—19398

HOXC10 promotes tumour metastasis by regulating the EMT-related gene Slug in ovarian cancer

Figure 2. HOXC10 accelerates OC cell migration. (A, B) Relative mRNA and protein expression levels of HOXC10. 8910 vs. PM cells. P=0.0177 (mRNA), P=0.0301 (protein). (C) Comparison of the migration ability of 8910 and PM cells via wound healing assays. P=0.0002. Scale bars, 200 μm. (D, E) Transfection efficiencies of the HOXC10 siRNA products. P<0.0001, P=0.0001 and P<0.0001; P=0.0008, P=0.0120 and P=0.0013. (F) Transwell assay of 8910 and PM cells transfected with HOXC10 siRNA products No. 1 and No. 3 and the negative control siRNA. 8910 cell graphs, P<0.0001 and P<0.0001. PM cell graphs, P<0.0001 and P<0.0001. Scale bars, 100 μm. (G, H) Wound healing assay of 8910 and PM cells transfected with HOXC10 siRNA products No. 1 and No. 3 and the negative control siRNA. P=0.0010 and P=0.0167; P=0.0025 and P=0.0097. Scale bars, 200 μm.