Research Paper Volume 12, Issue 19 pp 19399—19420

Identification of the EMT cell model based on TGF-β treatment. (A) Phase-contrast micrographs of Huh7 cells treated with 10 ng/ml TGF-β for 7 days or left untreated. Scale bar = 100 μm; (B) In vitro migration and invasion of Huh7 cells treated with 10 ng/ml TGF-β or left untreated. The number of migrated cells per field was counted after 48 h. (C) Relative mRNA levels of E-cadherin, ZEB1, SNAIL1, Twist and N-cadherin in Huh7 cells treated with 10 ng/ml TGF-β for 7 days or left untreated. (D) Relative protein levels of E-cadherin, ZEB1, SNAIL1, Twist and N-cadherin in Huh7 cells treated with 10 ng/ml TGF-β for 7 days or left untreated.