Research Paper Volume 12, Issue 20 pp 20366—20379

miR-140-3p inhibits bladder cancer cell proliferation and invasion by targeting FOXQ1

Figure 1. FOXQ1 promotes BCa cell proliferation and invasion. (A) Verification of FOXQ1 overexpression and knockdown in T24 and UMUC3 cells by western blot assay. Approximately 50 ug of protein was loaded into each lane 2 to 4 days after transfection. (B) T24 and UMUC3 cells were transfected with pWPI-FOXQ1-cDNA, pWPI-vector, pLKO-FOXQ1-shRNA or pLKO-vector. Cell growth was measured by MTT assay. (C) Transwell invasion assays were performed using UMUC3 cells (pWPI and oeFOXQ1) and T24 cells (pLKO and shFOXQ1). The invaded cells were counted in 10 randomly chosen microscopic fields (100X) of each experiment and pooled. (D) Analysis of microarray sequencing from NCBI GEO Datasets (GSE40355, GPL13497) showed the FOXQ1 mRNA level in 16 BCa and 8 nonmalignant bladder tissue samples. (E) Curves of BCa patient OS were analyzed according to FOXQ1 expression (data were download from TCGA). (F) FOXQ1 expression in 30 paired human primary BCa tissues and adjacent normal bladder tissues. (BD) Each sample was run in triplicate and used in multiple experiments to determine the mean ± SD. *P < 0.05; **P < 0.01 compared to controls.