Figure 3. miR-140-3p directly regulates FOXQ1 expression by targeting the 3'-UTR. (A) Sequence alignment of FOXQ1 3’-UTR with wild-type (WT) versus mutant potential miR-140-3p targeting sites using bioinformatics online databases (miRDB, Targetscan, and MicroCosm). (B, C) Co-transfection of wild-type or mutant seed regions of FOXQ1 3’-UTR constructs with miR-140-3p in T24 (B) and UMUC3 cells (C). The luciferase assay was applied to detect the luciferase activity. *P < 0.05 compared to controls.