Research Paper Volume 12, Issue 22 pp 22564—22581

Upregulated GDF-15 expression facilitates pancreatic ductal adenocarcinoma progression through orphan receptor GFRAL

Figure 6. GFRAL overexpression enhances the effects of GDF-15 in pancreatic cancer cells. (A) The efficiency of lentivirus infection was detected by fluorescence microscopy in AsPC-1; As(-) means Lv-NC, As(+) means Lv-GFRAL; (B) WB assay indicated that GFRAL expression was significantly upregulated in different pancreatic cancer cell lines(AsPC-1, Panc-1, Hs766t). (C, D) After GFRAL upregulation in AsPC-1 and SW1990 cells, pancreatic cancer cell proliferation ability was enhanced after coculture with 0ng/ml, 10ng/ml, or 20ng/ml GDF-15, with increasing concentrations of GDF-15, the proliferative ability also increased.