Research Paper Volume 12, Issue 22 pp 22582—22598

linc00968 inhibits the tumorigenesis and metastasis of lung adenocarcinoma via serving as a ceRNA against miR-9-5p and increasing CPEB3

Figure 3. linc00968 acts as a ceRNA to sponge miR-9-5p. (A) qRT-PCR analysis of linc00968 expression in A549 and H1975 cell. (B) RNA-FISH showed the subcellular localization of linc00968 in A549 and H1975 cell. linc00968 was stained with Cy3 (green) and nuclei were stained with DAPI (blue). (C) Prediction of miR-9-5p binding sites in the linc00968. (D) Ago2-RIP was performed in A549 and H1975 cell. (E) Luciferase activity in A549 and H1975 cell cotransfected with wt/mut linc00968 plasmid, and miR-9-5p mimic or miR-Ctrl. (F) Relative levels of miR-9-5p in A549 and H1975 cell transfected with si-linc00968 or scrambled control. (G) Relative levels of miR-9-5p in A549 and H1975 cell transfected with pcDNA3.1 or linc00968. **P<0.01 versus pcDNA3.1 or si-Ctrl.