Research Paper Volume 12, Issue 22 pp 22582—22598

linc00968 inhibits the tumorigenesis and metastasis of lung adenocarcinoma via serving as a ceRNA against miR-9-5p and increasing CPEB3

Figure 5. CPEB3 is a potential target of miR-9-5p. (A). Four bioinformatics methods (RNA22, Targetscan, PicTar and miRanda) were used predicted the targets of miR-9-5p. Prediction of miR-9-5p binding sites in the CPEB3. (B) Luciferase activity in A549 and H1975 cell cotransfected with wt/mut CPEB3 plasmid, and miR-9-5p mimic or miR-Ctrl. (C) Expression of CPEB3 in LUAD tissues and para-carcinoma tissues examined by qRT-PCR assay. **P<0.01 versus the para-carcinoma tissues. (D) Correlation analysis of linc00968 and CPEB3 in 56 cases of LUAD tissues. (E) Correlation analysis of CPEB3 and miR-9-5p in 56 cases of LUAD tissues. (F) The expression of CPEB3 was detected using western blot in miR-9-5p transfected A549 and anti-miR-9-5p transfected H1975 cell. (G) A549 and H1975 cell was transfected miR-9-5p or cotransfected with miR-9-5p and linc00968. (H) HCC827 was transfected anti-miR-9-5p or cotransfected with anti-miR-9-5p and si-linc00968. The expression of CPEB3 was assessed by western blot.