Figure 2. Protein and mRNA expression of TULA-2, SYK Y323 and VEGFR-2 Y1175 in ECs. ECs were incubated under normal culture conditions. (A) RT-qPCR showed no significant change in the mRNA levels of TULA-2 in OECs. (B) Western blot analysis revealed an increase in the protein levels of TULA-2 in OECs, and the phosphorylation levels of SYK Y323 and VEGFR-2 Y1175 were decreased in OECs (*P < 0.05, n = 3).