Research Paper Volume 12, Issue 22 pp 22599—22613

miR-25-3p promotes endothelial cell angiogenesis in aging mice via TULA-2/SYK/VEGFR-2 downregulation

Figure 4. The miR-25-3p inhibitor negatively regulates the angiogenic signaling pathway by reducing angiogenic growth factor expression. (A) RT-qPCR showed no significant change in the TULA-2 mRNA level in YECs after transfection with the miR-25-3p inhibitor. (B) Western blot analysis showed that the miR-25-3p inhibitor upregulated the protein level of TULA-2 and downregulated the phosphorylation levels of SYK Y323 and VEGFR-2 Y1175 in YECs (n=3, data are expressed as the mean ± SEM, *P < 0.05 versus the negative control).