Research Paper Volume 12, Issue 22 pp 22599—22613

miR-25-3p promotes endothelial cell angiogenesis in aging mice via TULA-2/SYK/VEGFR-2 downregulation

Figure 5. MiR-25-3p mimics positively regulate the angiogenic signaling pathway by increasing angiogenic growth factor expression. (A) RT-qPCR showed no significant change in the TULA-2 mRNA level in OECs after transfection with miR-25-3p mimics. (B) Western blot analysis showed that miR-25-3p mimics downregulated the protein level of TULA-2 and upregulated the phosphorylation levels of SYK Y323 and VEGFR-2 Y1175 in OECs (n=3, data are expressed as the mean ± SEM, *P < 0.05 versus the negative control).