Research Paper Volume 12, Issue 22 pp 22599—22613

miR-25-3p promotes endothelial cell angiogenesis in aging mice via TULA-2/SYK/VEGFR-2 downregulation

Figure 6. TULA-2 knockdown positively regulates angiogenesis in vitro and increases p-SYK Y323 and p-VEGFR-2 Y1175 expression at the protein level. (A) Transwell assays showed that TULA-2 siRNA promoted the migration ability of OECs, and co-transfection of OECs with both si-TULA-2 and miR-25-3p inhibitors did not suppress the effect of TULA-2 upregulation on EC migration relative to the effects of siRNA-NC (n=5; Scale bar, 100 μm). (B) Tube formation determined on Matrigel showed that TULA-2 siRNA increased the total tube length, and co-transfection of OECs with both si-TULA-2 and miR-25-3p inhibitors did not suppress the effect of TULA-2 upregulation on EC tube formation relative to the effects of siRNA-NC (n=4; scale bar, 100 μm). (C) Western blot analysis of relative TULA-2, SYK Y323 and VEGFR-2 Y1175 expression in OECs transfected with the negative control, si-TULA-2, si-TULA-2+ miR-25-3p inhibitor and miR-25-3p inhibitor (data are expressed as the mean ± SEM, *P < 0.05, **P < 0.01 versus the negative control).