Figure 7. The interaction of TULA-2 and SYK inhibits the phosphorylation of SYK Y323 and VEGFR-2 Y1175. (A) Western blot analysis of the Co-IP experiment showed that SYK immunoprecipitated with anti-TULA-2, SYK and TULA-2, which was visualized by Western blot. (B) Western blot analysis of relative TULA-2, SYK Y323 and VEGFR-2 Y1175 expression in YECs transfected with the control, BAY 61-3606 (SYK inhibitor), and BAY 61-3606 + si-TULA-2. The phosphorylation levels of SYK Y323 and VEGFR-2 Y1175 were decreased in YECs after transfection with BAY 61-3606 or BAY 61-3606 + si-TULA-2 (data are expressed as the mean ± SEM, *P < 0.05 versus the negative control).