Research Paper Volume 12, Issue 22 pp 22599—22613

miR-25-3p promotes endothelial cell angiogenesis in aging mice via TULA-2/SYK/VEGFR-2 downregulation

Figure 8. MiR-25-3p can promote ischemia-initiated blood flow recovery and angiogenesis in aged mice. (A) Representative images of laser Doppler blood flow before, immediately after, and 3, 7, 14, 21 and 28 days after femoral artery resection. (B) Blood flow in the ischemic hind limb was measured. The results are expressed as a ratio of the perfusion in the left limb (ischemic) to that in the right limb (control, nonischemic). The recovery of lower limb blood flow in the miR-25-3p agomir group (n=5) was better than that in the NC agomir group (n=5) and the PBS group (n=5) on the 7th, 14th and 28th days. (C) Representative images of anti-CD31 immunohistochemical sections of the gastrocnemius muscle of the left lower extremity. The expression level of CD31 in the miR-25-3p agomir group was higher than those in the NC agomir and PBS groups. For each animal, 5-6 randomly selected fields from 3-5 sections were counted (*P < 0.05, **P<0.01).