Research Paper Volume 12, Issue 20 pp 20396—20412

Figure 2. Knockdown of FAM83C-AS1 suppresses CRC progression. (A, B) Cell proliferation was evaluated by CCK-8 and colony formation assays. (C, D) Wound healing and transwell assays were carried out to measure cell migration and invasion abilities in different groups. (E) EMT process in transfected cells was analyzed using western blot and immunofluorescence analyses. E-cadherin and N-cadherin were found to be EMT-related proteins. (F) The sphere formation ability of transfected cells was assessed using sphere formation assay. (G) Cell stemness in different groups was analyzed through flow cytometer. CD133⁺ or CD44⁺ was utilized as stem cell marker and was related to cell stemness. ^**P < 0.01.