Priority Research Paper Volume 12, Issue 19 pp 18804—18821

Evolutionary feature analysis of the slc4a2b gene. (A) Evolutionary relationships of SLC4A2 in different species. The evolutionary history was inferred using the Neighbor-Joining method, and the optimal tree with the sum of branch length = 1.66619414 is shown. The tree is drawn to scale, with branch lengths in the same units as those of the evolutionary distances used to infer the phylogenetic tree. The evolutionary distances were computed using the Poisson correction method and are in the units of the number of amino acid substitutions per site. The analysis involved 14 amino acid sequences. All positions containing gaps and missing data were eliminated. There were a total of 237 positions in the final dataset. The evolutionary analysis was conducted in MEGA6. (B) slc4a2b phyloP8way conservation score (vertical red line) and the zebrafish protein coding gene phyloP8way conservation score density distribution (black line). The x-axis is the average phyloP8way score for the coding sequence region of the gene, and the y-axis is the density of the number of genes in each conservation score range.