Research Paper Volume 12, Issue 18 pp 18561—18570

Chetomin rescues pathogenic phenotype of LRRK2 mutation in drosophila

Figure 1. LRRK2 interacts and phosphorylates PRDX2. (A) LRRK2-GFP and PRDX2-flag are co transfected in HEK293T cells. The lysates were collected and subjected to immunoprecipitation with anti-GFP antibody. The protein was subjected to western blot with anti-flag. Un-transfected cells, LRRK2 GFP alone and PRDX flag alone was transfected into HEK293T cells to serve as negative control. (B) Endogenous colocalization of LRRK2 (green) and PRDX2 (red) protein in SKNSH neuronal cells (C) In vitro kinase assay on SDS-PAGE gel show phosphorylation of PRDX2 by LRRK2 wildtype and G2019S.