Research Paper Volume 12, Issue 20 pp 20445—20456

Dual inhibition of DNA-PKcs and mTOR by CC-115 potently inhibits human renal cell carcinoma cell growth

Figure 1. CC-115 inhibits human RCC cell survival and proliferation. Established human RCC cell lines (786-O and A498), the primary human RCC cells (derived from two patients, “RCC1/2”), immortalized HK-2 tubular epithelial cells as well as the primary human renal epithelial cells (“Pri-Epi”) were treated with indicated concentration of CC-115 for the applied time periods, cell viability was tested by CCK-8 assay (A, D); Cell proliferation was tested by soft agar colony formation assay (B) and the BrdU ELISA assay (C, E). Data were expressed as mean ± standard deviation (SD, same for all Figures). * P < 0.05 vs. untreated control group (“Ctrl”). All in vitro experiments were repeated 3-4 times, and similar results were obtained.