Research Paper Volume 12, Issue 20 pp 20445—20456

Dual inhibition of DNA-PKcs and mTOR by CC-115 potently inhibits human renal cell carcinoma cell growth

Figure 2. CC-115 induces apoptosis activation in human RCC cells. Established human RCC cell lines (786-O and A498), the primary human RCC cells (“RCC1/2”), HK-2 tubular epithelial cells and the primary human renal epithelial cells (“Pri-Epi”) were treated with indicated concentration of CC-115 for the applied time periods, cell apoptosis was tested by the assays mentioned in the text (A, B, D); For (C), 786-O cells were pretreated for 30 min with 50 μM of z-VAD-fmk (“zvadfmk”) or z-DEVD-fmk (“zdevdfmk”) before CC-115 treatment, and cell viability analyzed by CCK-8 assay. *P < 0.05 vs. untreated control group (“Ctrl”). #P < 0.05 vs. “dmso” (0.5% DMSO) (C).