Research Paper Volume 12, Issue 21 pp 21253—21272

Regulation of the regenerative activity of dental pulp stem cells from exfoliated deciduous teeth (SHED) of children by TGF-β1 is associated with ALK5/Smad2, TAK1, p38 and MEK/ERK signaling

Figure 4. Effect of TGF-β1 on the growth of SHED. (A) Number of viable SHED after exposure to TGF-β1 for 5 days as estimated by MTT assay. Results were expressed as cell viability (% of control, Mean ± SE). (B) Effect of SB431542 on the TGF-β1-induced growth of SHED as analyzed by MTT assay. (C) Effect of 5z-7oxozeaenol on the TGF-β1-induced growth of SHED as analyzed by MTT assay. (D) Effect of U0126 on the TGF-β1-induced growth of SHED as analyzed by MTT assay. (E) Effect of SB203580 on the TGF-β1-induced growth of SHED as analyzed by MTT assay. *Denotes statistically significant difference when compared to control, #denotes statistically significant difference when compared with TGF-β1 (5 ng/ml)-treated group.