Research Paper Volume 12, Issue 21 pp 21253—21272

Regulation of the regenerative activity of dental pulp stem cells from exfoliated deciduous teeth (SHED) of children by TGF-β1 is associated with ALK5/Smad2, TAK1, p38 and MEK/ERK signaling

Figure 7. Effect of TGF-β1 on the ALP activities of cultured SHED as analyzed by ALP staining. (A) ALP staining of SHED after exposure to TGF-β1 for 5 days. (B) Effect of SB431542 on the TGF-β1-induced increase or decrease in ALP activity of SHED. (C) Effect of 5z-7oxozeaenol on the TGF-β1-induced increase or decrease in ALP activity of SHED. (D) Effect of U0126 on the TGF-β1-induced increase or decrease in ALP activity of SHED. (E) Effect of SB203580 on the TGF-β1-induced increase or decrease in ALP activity of SHED. One representative ALP staining result was shown.