Figure 6. The affinity of targeting peptides increased with the growing of concentration and could be blocked by HLA-E blocking antibody. (A) 293T cells did not express HLA-E, and plasmid transfect 293T cells expressed HLA-E (p<0.01). (B) the affinity of model peptide and PEPTIDE 1-3 increased with the increasing of concentration. Precultured transfected 293T cells with HLA-E blocking antibody, the affinity of P3 disappeared. (C) The statistical chart about the affinity of peptides to HLA-E. All repeated for three times.