Figure 3. L-dopa and SAM treatment shows opposing effects on the angiogenesis and cell proliferation. (A and B) After treatment with the indicated concentrations of L-dopa or SAM for 24 h, endothelial tube formation was assessed using light microscopy. The average number of microtubules in 3 random horizons was analyzed using ImageJ software. (C) Cells were treated with L-dopa for 24 h and were incubated for another 2 weeks before fixation, staining and colony quantification. Clonogenic assays were performed in triplicate. (D) CCK-8 assay was using to evaluate the effect of L-dopa on the cell viability. (E) Colony formation assays show the effect of SAM on primary HUVECs. The quantitative results shown of three independent experiments are the mean ± SD. (F) CCK-8 assay was used to evaluate the effect of SAM on the cell viability. The asterisk (* or **) indicates a significant (p < 0.05 or p < 0.01, respectively) compared with control groups..