Research Paper Volume 12, Issue 21 pp 21290—21307

S-adenosylmethionine administration inhibits levodopa-induced vascular endothelial growth factor-A expression

Figure 4. SAM suppresses the proliferation and angiogenesis effect of L-dopa in primary-HUVECs. (A) Primary HUVECs were treated with a single or combination agent for 24 h and then analyzed with tube-formation assay. The average number of microtubules from three experiments was analyzed using ImageJ software. (B) After treatment with the indicated conditions, cell viability was evaluated using the CCK-8 assay. The experiments were repeated for three independent times. And the quantitative results shown are means ± SD. The asterisk (* or **) indicates a significant (p < 0.05 or p < 0.01, respectively) compared with control groups.