Figure 6. Effects of SAM on the cell cycle distribution in Primary-HUVECs. (A) Upon treatment with SAM at 0.04 mM and 0.08 mM for 24 h, the cell-cycle progression of primary HUVECs was analyzed by flow cytometry. (B) Flow cytometry was used to assess the cell cycle in primary HUVECs treated by L-dopa at 5 μM and 15 μM for 24 h. (C) Cell cycle-related proteins (CDK4/6 and p16) were determined using western blot analysis. α-Tubulin was used for the loading control. The quantitative results shown of three independent experiments are means ± SD.