Research Paper Volume 12, Issue 22 pp 22656—22687

Long non-coding RNA ZFAS1 promotes colorectal cancer tumorigenesis and development through DDX21-POLR1B regulatory axis

Figure 2. The effect of lncRNA ZFAS1 on CRC cell biological characteristics by regulating DDX21. (A and B) The expression of lncRNA ZFAS1 after overexpression or silencing lncRNA ZFAS1 in SW620 and SW480 cells by RT-PCR (A) and qPCR assay (B). (C and D) The mRNA and protein levels of DDX21 expression after interfering lncRNA ZFAS1 expression in both SW620 and SW480 cells detected by qPCR assay (C), and western blot (D). (E) Cell number monitoring assays showed the cell proliferative variation after ectopic or knockdown lncRNA ZFAS1 in SW620 and SW480 cells. (F) Cell colony formation assays were performed to identify the cell cloning capability upon lncRNA ZFAS1 silencing or overexpressing in SW620 and SW480 cells. n = 3 independent experiments. (G) The percentage (%) of cell apoptosis was detected upon lncRNA ZFAS1 overexpressing or silencing in SW620 and SW480 cells by Flow cytometry. n = 3 independent experiments. (H) The migration ability was determined after ectopic or knockdown lncRNA ZFAS1 in SW620 and SW480 cells. n = 3 independent experiments. Data were shown as mean ± s.d.. * P <0.05; **P <0.01; *** P <0.001; **** P <0.0001.